Includes bibliographical references and index.

Physical analysis of chromosome size variation -- Genetic mapping in Salmonella enterica -- Insertion sequences as genomic markers -- The use of noncoding microsatellite length analysis for bacterial strain typing -- How to amplify easily, on the bacterial chromosome, a desired DNA sequence -- Generalized transduction -- Use of conditional-replication, integration, and modular CRIM plasmids to make single-copy lacZ fusions -- Genetic footprinting for bacterial functional genomics -- Gene transfer to plants through bacterial vectors -- Quorum sensing: approaches to identify signals and signalling genes in gram-negative bacteria -- Transcriptional profiling in bacteria using microarrays -- Transcriptome analysis by macroarrays -- Prokaryotic proteomics -- Intein-mediated protein purification -- Two-hybrid assay in Escherichia coli K12.