Genetic analysis and marker trait association for Powdery mildew resistance in mutagenized and biparental population of pea (Pisum sativum) /
Pheirim, Reginah
Genetic analysis and marker trait association for Powdery mildew resistance in mutagenized and biparental population of pea (Pisum sativum) / Reginah Pheirim. - Umiam ; CPGS-AS, CAU , March 2022. - xiv, 118p.: ill., some col.; 30 cm. - [Genetics and Plant Breeding, School of Crop Improvement] .
Abstract:
Field pea (Pisum sativum L., 2n = 14) is one of the most important pulse crops grown worldwide. Several biotic and abiotic stress constraints in achieving potential production of field pea and powdery mildew (Erysiphe pisi) is one of the major constraint causing yield reductions up to 26– 47%. Most demanding local variety of Manipur, Makhyatmubi for its taste, higher yield and high protein content could not be cultivated widely because of its tall nature and high susceptibility to biotic stresses including powdery mildew. Availability of genetic variation is an essential prerequisite for genetic improvement of crops. So, mutation was induced by treating Makhyatmubi with NaN3 (Sodium azide) and hybridized Makhyatmubi with Prakash, which is a well known powdery mildew resistant variety in India, possessing several other contrasting characters. From this, three different populations consisting of 60 M3 families, 132 F2 individuals and 71 F3 families were evaluated for yield and powdery mildew reaction in the present study. Analysis of variance was observed to have highly significant variation in both mutants as well as segregating populations (F2 and F3) for almost all the characters under study. High Genotypic Coefficient Variation (GCV: 61.66%) and Phenotypic Coefficient Variation (PCV: 64.29%) were recorded for primary branches per plant while heritability coupled with high genetic advance as per cent over mean (GAM) were recorded for primary branches per plant (91.66%, 121.81%), number of pods per plant (97.17%, 68.42%) and seed yield per plant (96.41%, 68.94%) for the mutant lines in M3 generation. Number of pods per plant and seed yield per plant showed high GCV, PCV, heritability and GAM in segregating populations (F2 and F3).This indicated that selection based on such characters would be rewarding. The correlation analysis with highest positive significant correlation with seed yield per plant was number of pods per plant (0.95). Screening of powdery mildew revealed mutant line M-144 as moderately resistant to powdery mildew which also showed high seed yield performance. In F2 progenies, 98 susceptible and 34 resistant plants which phenotypically fitted to a segregation ratio of 3:1 (susceptible vs resistant) was recorded indicating recessive nature of inheritance. MP-13, MP-109, MP-5(2) and MP-119 (2) showed superior performance in F3 generation on the basis of seed yield as well as powdery mildew disease resistant. The latter two individuals, MP-5(2) and MP-119 (2) were also recorded to have exhibited superior performance in several other characters under study. The progenies from the cross of the two parents served as mapping population in the present study for marker trait association for powdery mildew resistant gene (er1). Parental polymorphisms were surveyed by a total of 112 SSR markers spanning all seven chromosomes of the field pea. Out of 112 SSR markers, 30 markers were found to be polymorphic. The polymorphic markers were used for surveying markers associated to powdery mildew using BSA (Bulk Segregant Analysis) method. The result of BSA depicted SSR marker A5 to be associated with powdery mildew. Further analysis by Simple Linear Regression analysis (R2 value of 0.67) for the entire F2 population confirmed its association with the powdery mildew. The chi square test for the allele of the A5 marker resulted a ratio fitted to 1:2:1 (ERER:Erer: erer). Therefore, this marker can be used to identify and employed for marker assisted selection for powdery mildew in pea. This study also indicated that resistant to powdery mildew in Prakash variety may be due to er1 gene. The easily scorable polymorphic markers were also analyzed for yield and its related characters under study. The results revealed that marker AA122 showed R2 value of 0.39 for number of pods per plant and R2 value of 0.36 for seed yield per plant and marker AB136 exhibited 0.60 R2 value for number of seeds per pod.
Peas--Genetic analysis.
Peas--Pisum sativum.
Plant diseases--Powdery mildew--Resistance.
635.656233
Genetic analysis and marker trait association for Powdery mildew resistance in mutagenized and biparental population of pea (Pisum sativum) / Reginah Pheirim. - Umiam ; CPGS-AS, CAU , March 2022. - xiv, 118p.: ill., some col.; 30 cm. - [Genetics and Plant Breeding, School of Crop Improvement] .
Abstract:
Field pea (Pisum sativum L., 2n = 14) is one of the most important pulse crops grown worldwide. Several biotic and abiotic stress constraints in achieving potential production of field pea and powdery mildew (Erysiphe pisi) is one of the major constraint causing yield reductions up to 26– 47%. Most demanding local variety of Manipur, Makhyatmubi for its taste, higher yield and high protein content could not be cultivated widely because of its tall nature and high susceptibility to biotic stresses including powdery mildew. Availability of genetic variation is an essential prerequisite for genetic improvement of crops. So, mutation was induced by treating Makhyatmubi with NaN3 (Sodium azide) and hybridized Makhyatmubi with Prakash, which is a well known powdery mildew resistant variety in India, possessing several other contrasting characters. From this, three different populations consisting of 60 M3 families, 132 F2 individuals and 71 F3 families were evaluated for yield and powdery mildew reaction in the present study. Analysis of variance was observed to have highly significant variation in both mutants as well as segregating populations (F2 and F3) for almost all the characters under study. High Genotypic Coefficient Variation (GCV: 61.66%) and Phenotypic Coefficient Variation (PCV: 64.29%) were recorded for primary branches per plant while heritability coupled with high genetic advance as per cent over mean (GAM) were recorded for primary branches per plant (91.66%, 121.81%), number of pods per plant (97.17%, 68.42%) and seed yield per plant (96.41%, 68.94%) for the mutant lines in M3 generation. Number of pods per plant and seed yield per plant showed high GCV, PCV, heritability and GAM in segregating populations (F2 and F3).This indicated that selection based on such characters would be rewarding. The correlation analysis with highest positive significant correlation with seed yield per plant was number of pods per plant (0.95). Screening of powdery mildew revealed mutant line M-144 as moderately resistant to powdery mildew which also showed high seed yield performance. In F2 progenies, 98 susceptible and 34 resistant plants which phenotypically fitted to a segregation ratio of 3:1 (susceptible vs resistant) was recorded indicating recessive nature of inheritance. MP-13, MP-109, MP-5(2) and MP-119 (2) showed superior performance in F3 generation on the basis of seed yield as well as powdery mildew disease resistant. The latter two individuals, MP-5(2) and MP-119 (2) were also recorded to have exhibited superior performance in several other characters under study. The progenies from the cross of the two parents served as mapping population in the present study for marker trait association for powdery mildew resistant gene (er1). Parental polymorphisms were surveyed by a total of 112 SSR markers spanning all seven chromosomes of the field pea. Out of 112 SSR markers, 30 markers were found to be polymorphic. The polymorphic markers were used for surveying markers associated to powdery mildew using BSA (Bulk Segregant Analysis) method. The result of BSA depicted SSR marker A5 to be associated with powdery mildew. Further analysis by Simple Linear Regression analysis (R2 value of 0.67) for the entire F2 population confirmed its association with the powdery mildew. The chi square test for the allele of the A5 marker resulted a ratio fitted to 1:2:1 (ERER:Erer: erer). Therefore, this marker can be used to identify and employed for marker assisted selection for powdery mildew in pea. This study also indicated that resistant to powdery mildew in Prakash variety may be due to er1 gene. The easily scorable polymorphic markers were also analyzed for yield and its related characters under study. The results revealed that marker AA122 showed R2 value of 0.39 for number of pods per plant and R2 value of 0.36 for seed yield per plant and marker AB136 exhibited 0.60 R2 value for number of seeds per pod.
Peas--Genetic analysis.
Peas--Pisum sativum.
Plant diseases--Powdery mildew--Resistance.
635.656233