Inter-specific hybridisation and embryo rescue in Capsicum / (Record no. 4018)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02726nam a2200229Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | OSt |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20220103120419.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 130502s9999 xx 000 0 und d |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CPGS |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 633.8423 |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Debbarma, Chandan |
| 9 (RLIN) | 913 |
| 110 ## - MAIN ENTRY--CORPORATE NAME | |
| Corporate name or jurisdiction name as entry element | V.K.Khanna |
| 9 (RLIN) | 914 |
| 245 ## - TITLE STATEMENT | |
| Title | Inter-specific hybridisation and embryo rescue in Capsicum / |
| Statement of responsibility, etc | by Chandan Debbarma |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication, distribution, etc | Umiam : |
| Name of publisher, distributor, etc | CPGS, CAU |
| Date of publication, distribution, etc | c2011 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | [38], 114p. : |
| Other physical details | ill., some col. ; |
| 440 ## - SERIES STATEMENT/ADDED ENTRY--TITLE | |
| Title | [Plant Molecular Biology and Biotechnology, School of Crop Improvement] |
| 9 (RLIN) | 915 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc | Abstract:<br/><br/>Chilli belongs to the genus Capsicum, which is among the world's extensively grown spice crop. India ranks first with an average yield of 1.6 mt ha-1 from the total cultivated area of 7.67 lakhs ha. In Meghalaya, it is the third most important spice crop after ginger and turmeric with an area of 1900 hectares and production 2300 tonnes. However, diseases namely, tobacco mosaic virus (TMV), root rot, tomato spotted wilt virus (TSWV) etc, leads to a considerable yield decline. It is therefore necessary to transfer the important genes for various characters from one species to another. But there has been very little success due to problem in crossability.The present investigation was carried out to determine as to whether this low success percent is due to pre-fertilization or post fertilization problem and optimum timing for embryo rescue, standardization of hormonal concentration for embryo rescue to get hybrid plant, and hybridity test through morphological and DNA markers viz. RAPD.In the crossability studies, out of the three species (viz. Capsicum annuum, Capsicum chinense and Capsicum frutescens) two varieties of Capsicum annuum, viz. Pusa Jwala and Kashi Anmol, showed higher percent of fruits set when used as female parents while Capsicum chinense and Capsicum frutescens showed no success. Similar results were observed for pollen viability, pollen germination and pollen tube growth.In in-vitro investigation of embryo rescue, the optimum timing for explants collection was found to be 27-33 days after pollination (DAP). The highest percentage of embryo growth was observed with the hormone concentration of 0.5 mg l-1 GA3 and 0.05 mg l-1 of NAA.Hybridity test was confirmed with morphological markers and RAPD markers. Two bands (900 bp each) unique to Capsicum annuum have been identified in RAPD markers OPV-12 and OPZ-4 which confirm the hybridity of the progeny in Capsicum chinense x Capsicum annuum and Capsicum frutescens x Capsicum annuum cross. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Chilli-Hybridisation |
| 9 (RLIN) | 916 |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Capsicum |
| General subdivision | Genetics |
| 9 (RLIN) | 917 |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Khanna, V. K. |
| Relator term | Major Advisor |
| 9 (RLIN) | 918 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | MSc Thesis |
| Withdrawn status | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection code | Home library | Current library | Date acquired | Total Checkouts | Full call number | Barcode | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | School of Crop Improvement | CPGS | CPGS | 02/05/2013 | 633.8423 DEB | TH065 | 24/01/2020 | 02/05/2013 | MSc Thesis |