Identification and cloning of cold responsive genes in rice / (Record no. 4094)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02928nam a22002177a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | OSt |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20220103120530.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 130823b xxu||||| |||| 00| 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CPGS |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 633.1823 |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Bhutia, Tshering Chomu |
| 9 (RLIN) | 1192 |
| 245 ## - TITLE STATEMENT | |
| Title | Identification and cloning of cold responsive genes in rice / |
| Statement of responsibility, etc | by Tshering Chomu Bhutia |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication, distribution, etc | Umiam : |
| Name of publisher, distributor, etc | CPGS, CAU |
| Date of publication, distribution, etc | c2012 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | [34], 59p.: |
| Other physical details | ill., some col.; |
| 440 ## - SERIES STATEMENT/ADDED ENTRY--TITLE | |
| Title | [Plant Molecular Biology and Biotechnology,School of Crop Improvement] |
| 9 (RLIN) | 1193 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc | Rice is one of the most important cereal crops of high economic and social value feeding the population of about 6.8 billion people over the world. In the past decade, changes such as rapid economic growth, especially in parts of Asia, rising wage rates, increasing diversification of diets, global climate change, and a greater integration of the food economy with other sectors of the global economy, including both energy and financial markets, have converged to shape the way rice is produced today and will be produced in the future. One of the main constraint affecting the yield are abiotic and biotic stresses like drought, salt, cold stresses and diseases like blast. These affect both yield and plant growth Cold stress affects the rice crop at seedling, reproductive and ripening stages. Cold tolerance at these stages mainly seedling stage is an important parameter to reduce the rice productivity in NEHR. <br/>Work at molecular level on the cold responsive genes has revealed role of genes, transcription factors (T 8) and quantitative trait loci (OTL ). Present study was carried out to find the novel genes, to validate the responsiveness in two rice landraces (UR5 and UR 17-2) adapted to low temperature by RT-PCR method and clone the selected genes into an appropriate vector. The genes were selected from the referred paper which provided 714 unregulated novel genes. Out of 10 selected genes, 6 showed amplification when standardized with genomic DNA. RT-PCR experiment showed that at 24 hours stress 2 genes (OsZim and OsFbx257) show differential expression. While at 3 hours 4 genes viz, OsPs, OsFbx257, OsZim and OsPal were differentially regulated. Based on RT-PCR data 4 genes (OsPal, Oszim, OsFbx257 and OsPs) were selected for cloning in TOPO-TA vector. The genes selected for cloning belong to different gene families viz, ZIM, TIGR01615, phenylalanine lyase and F-box. The OsFbx257 gene was successfully cloned. This gene could be evaluated further for its role in planta under low temperature. The role of ZIM, TIGRR1615 and Phenylalanine lyase like proteins in response to low temperature is not very well worked out in rice. These could be future research areas of interest. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Rice |
| General subdivision | Identification and cloning |
| 9 (RLIN) | 1194 |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Rice-Cold Responsive genes |
| 9 (RLIN) | 1195 |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Tyagi, Wricha |
| Relator term | Major Advisor |
| 9 (RLIN) | 1196 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | MSc Thesis |
| Withdrawn status | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection code | Home library | Current library | Date acquired | Total Checkouts | Full call number | Barcode | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | School of Crop Improvement | CPGS | CPGS | 23/08/2013 | 633.1823 BHU | TH094 | 24/01/2020 | 23/08/2013 | MSc Thesis |