Molecular based screening of native Bt isolates of Manipur and their bioactivity against cabbage butterfly, Pieris brassicae (Linnaeus) / (Record no. 5612)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03237nam a22002177a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | OSt |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20240301122856.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 240301b ||||| |||| 00| 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CPGS |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Mathew, Manju |
| 9 (RLIN) | 10012 |
| 245 ## - TITLE STATEMENT | |
| Title | Molecular based screening of native Bt isolates of Manipur and their bioactivity against cabbage butterfly, Pieris brassicae (Linnaeus) / |
| Statement of responsibility, etc | Manju Mathew. |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication, distribution, etc | Umiam : |
| Name of publisher, distributor, etc | CPGSAS, CAU-I, |
| Date of publication, distribution, etc | October 2023. |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | [69p. : |
| Other physical details | ill., some col.; |
| Dimensions | 30cm. |
| 440 ## - SERIES STATEMENT/ADDED ENTRY--TITLE | |
| Title | [Entomology, School of Crop Protection] |
| 9 (RLIN) | 9081 |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE | |
| Bibliography, etc | Includes bibliographical references. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc | Cole crops are major vegetable crops of Meghalaya contributing significantly to the agriculture of the state. However, the cabbage butterfly, Pieris brassicae is an important pest of the cole crops causing a significant loss by diminishing the production as well as market value of the produce. Therefore, the management of this pest is crucial for which the adoption of Bacillus thuringiensis is an effective, ecofriendly and sustainable approach as compared to the conventional methods. Bt is a naturally occurring gram positive, rod shaped, motile, facultative anaerobic and also spore forming bacterium found in soil which is widely used as an eco-friendly biopesticide against insect pests in agriculture and forestry. The bacterium is renowned for its high specificity and environmental safety. In the present investigation, the isolation and characterisation of diverse isolates of Bt from Manipur region and the evaluation and shortlisting of novel Bt isolates against Cabbage butterfly, Pieris brassicae (Linnaeus) were undertaken. A total of 10 Bt strains were isolated from the Manipur region. Among them, two strains each from the districts of Bishnupur, Kakching and Noney and one each from the districts of Churachandpur, Kangpokpi, Senapati and Tamenglong were isolated. The isolates exhibited the characteristic creamy white colour, elevated or flat colonies with dry and rough texture. The phase contrast and electron microscopy studies revealed the presence of crystals with smooth spherical, spherical with undulating surface and deflated balloon like structures. The cry gene content of the isolates was detected by PCR analysis revealing the presence of cry1, cry1A and cry1IA in them. The bioassay studies have resulted in four isolates (MKAKG-1, MBNPR-1, MBNPR-2 and MSNPT-1) which exhibited 100% mortality in the P. brassicae larvae. When compared to the reference strains, two of the novel isolates viz., MKAKG-1 and MBNPR-1 exhibited higher mortality. The median lethal concentrations of these isolates are 2.8×105 (95% FL, 5.5×104 -1.4×106) and 3.0×105 (95% FL, 5.8×104 - 1.6×106) respectively. From the present study, it can be concluded that MKAKG-1 and MBNPR-1 are potential Bt strains that can be used effectively against P. brassicae. These isolates can be further used for developing and producing new active bioinsecticides which can be integrated with the management program of lepidopteran pests. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Pest management |
| General subdivision | Cole crops. |
| 9 (RLIN) | 10013 |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Pieris brassicae |
| General subdivision | Control. |
| 9 (RLIN) | 10014 |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ningthoujam, Kennedy |
| Relator term | Major Advisor. |
| 9 (RLIN) | 9083 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | MSc Thesis |
| Withdrawn status | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection code | Home library | Current library | Date acquired | Total Checkouts | Barcode | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | Not For Loan | School of Crop Protection | CPGS | CPGS | 13/02/2024 | TH525 | 07/03/2024 | 01/03/2024 | MSc Thesis |