Efficacy of Silver (Ag) and Gold (Au) nanoparticles against wilt of potato caused by Ralstonia solanacearum Smith / (Record no. 5715)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03544nam a22002417a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | OSt |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250306151320.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250306b ||||| |||| 00| 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CPGS |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Raj, Rajni |
| 9 (RLIN) | 10318 |
| 245 ## - TITLE STATEMENT | |
| Title | Efficacy of Silver (Ag) and Gold (Au) nanoparticles against wilt of potato caused by Ralstonia solanacearum Smith / |
| Statement of responsibility, etc | Rajni Raj. |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication, distribution, etc | Umiam : |
| Name of publisher, distributor, etc | CPGSAS, CAU(Imphal), |
| Date of publication, distribution, etc | September 2024. |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 92p. |
| 440 ## - SERIES STATEMENT/ADDED ENTRY--TITLE | |
| Title | [Plant Pathology, School of Crop Protection] |
| 9 (RLIN) | 9105 |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE | |
| Bibliography, etc | Includes bibliographical references. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc | Bacterial wilt of potato (Solanum tuberosum L.) caused by Ralstonia solanacearum Smith is difficult to management due to its soil-borne nature and has a wide host range. In the present study efficacy of two nanoparticles (NPs) viz., silver (Ag) and gold (Au) were tested both in vitro and in vivo against the R. solanacearum. In in vitro seven different doses were tested against the pathogen by disc diffusion method and result showed that, the effect was significantly dependant on the concentration of the NPs. The highest inhibition zone (mm) was recorded for AgNPs and AuNPs at 5000 ppm which was 1.70 mm and 1.45 mm respectively. Scanning Electron Microscope (SEM) analysis on action of AgNPs and AuNPs at 5000 ppm on bacterial cells depicts the distortion of the cell wall of NPs treated bacterial cell. ROS (reactive oxygen species) formation was more prominent in AgNPs and AuNPs treated bacterial cell. The effect of AgNPs and AuNPs was tested by seed treatment and soil application in both pot and field conditions observation on effect of NPs on plant growth parameters, seed germination percentage, and per cent disease incidence of potato plants were recorded with challenged inoculation of R. solanacearum. Results demonstrated that in pot condition, an increased plant height up to 43.83 cm as compared to control (37.37 cm) was recorded 60 days after sowing along with the per cent wilt disease reduction (70%) over control. In the AuNPs treated pot an yield of 2.5 kg per plant was recorded when AuNPs were used as treatment. Whereas in control an yield of 0.73 kg per plant was recorded. Similarly, in field conditions, an increased plant height up to 20.63 cm as compared to control (15.87 cm) was recorded at 60 days after sowing along with the per cent bacterial wilt disease incidence (29.63%). An yield of 4.72 kg per plot was recorded which was found much higher than the yield of control (2.92 kg per plot). The results obtained for the biochemical tests for the Ag and Au NPs treated plot at 5000 ppm resulted increase of TSS (187.41 ppm) for AgNPs, TSP (440.45 ppm) for control (with R. solanacearum), phenol (253.01 ppm) content for AgNPs, flavonoids (862 ppm) for both AgNPs, terpenoid (21.92 mg) for control (with R. solanacearum) and proline content (100.36 mg g-1) for AuNPs. Soil microbial population in Ag and Au NPs treated plot was found to be lowest in case of treatment with AgNPs at 5000 ppm, 3000 ppm and AuNPs at 5000 ppm. The present study showed encouraging result of using Ag and Au NPs as seed treating agent and soil drenching agent for the management of bacterial wilt of potato with enhance plant defence metabolite and yield. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Potato |
| General subdivision | Wilt disease |
| -- | Management. |
| 9 (RLIN) | 10319 |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Silver Nanoparticles |
| 9 (RLIN) | 10317 |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Gold Nanoparticles. |
| 9 (RLIN) | 10320 |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Dutta, Pranab |
| Relator term | Major Advisor. |
| 9 (RLIN) | 5806 |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://krishikosh.egranth.ac.in/handle/1/5810220780">https://krishikosh.egranth.ac.in/handle/1/5810220780</a> |
| Link text | Online. |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | MSc Thesis |
| Withdrawn status | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection code | Home library | Current library | Date acquired | Total Checkouts | Barcode | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | Not For Loan | School of Crop Protection | CPGS | CPGS | 29/01/2025 | TH572 | 06/03/2025 | 06/03/2025 | MSc Thesis |