000			02826nam a22002297a 4500
003			OSt
005			20241008113924.0
008			241008b ||||| |||| 00| 0 eng d
040			_cCPGS
100			_aAustin, Hans _910160
245			_aEvaluation of antifungal activity of bacterial endophytes of legumes against White Stem Rot of peanut caused by Sclerotium rolfsii Sacc. / _cHans Austin
260			_aUmiam : _bCPGSAS, CAU, _cMay 2024.
300			_a99p. : _bill., some col.;
440			_a[Plant Pathology, School of Crop Protection] _99105
504			_aIncludes bibliographical references.
520			_aWhite stem rot of peanuts caused by Sclerotium rolfsii Sacc., a destructive pathogen with a broad host range that affects various crops and plants, poses a significant threat worldwide by causing yield losses of up to 40 % for peanuts. Our study aims to investigate the antifungal potential of root endophytes against Sclerotium rolfsii. The pathogen was isolated, purified and characterized by cultural, morphological and molecular studies and the association was confirmed by pathogenicity test. Root endophytes of different leguminous plants like peanut, chickpea, faba bean and pea was isolated and screened in in vitro against Sclerotium rolfsii. Among the 33 root endophytes tested, three isolates exhibited the highest inhibition to mycelial growth and sclerotia germination inhibition against the pathogen: CE2 (Bacillus amyloliquifaciens) with of 86.59 % and 86.66 %, CE5 (Burkholderia ambifaria) with 74.07 % and 80.00 %, and PE2 (Burkholderia plantari) 55.92 % and 76.66 %, respectively. These potential endophytes were chosen for field and pot experiments based on antagonistic assays and their promising growth promotion activity in in vitro conditions. Three individual strains (T1:CE2, T2:PE2, T3:PE2) and four consortia (T4:CE2+CE5, T5:CE5+PE2, T6:CE2+PE2, T7:CE2+CE5+PE2) of root endophytes were then tested in field and all treatments resulted in significant disease suppression. Among the treatments, CE2+CE5+PE2 (T7) showed highest reduction in disease severity of 80.04 % over the control followed by CE1+PE2 (T6) of 71.96 %. Whereas, individual isolate PE2 showed the disease reduction of 45.25 %. Pod and kernel yield were also found high in CE2+CE5+PE2 (T7), with an average yield of 3.64 and 3.02 t/ha respectively. These findings appear to offer a viable bio-control alternative for managing white stem rot of peanuts and opening avenues for resilient and sustainable strategies in protecting crops against this destructive fungal pathogen.
650			_aPeanuts _xWhite stem rot. _910161
650			_aRoot endophytes _xSclerotium rolfsii _910162
700			_aT. Rajesh _eMajor Advisor. _96144
856			_uhttps://krishikosh.egranth.ac.in/handle/1/5810215345 _yOnline.
942			_2ddc _cTH
999			_c5663 _d5663