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| 005 | 20250305110434.0 | ||
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| 040 | _cCPGS | ||
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_aOinam Bombayshwori Devi _910232 |
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_aDevelopment of microbial NPKZn biofertilizer consortium for use in acid soil / _cOinam Bombayshwori Devi |
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_aUmiam : _bCPGSAS, CAU(Imphal), _cAugust 2024. |
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| 300 | _a96p. | ||
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_a[Soil Science and Agriculture Chemistry, School of Natural Resource Management] _99147 |
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| 504 | _aIncludes bibliographical references. | ||
| 520 | _aScientific research has clearly established the role of microbes in crop growth and development. By altering soil microbes in rhizosphere, plant-microbe interaction has been found to be influenced, thereby increasing plant yield. Different beneficial bacteria are broadly categorized into many groups in soil viz., nutrient mobilizers, nitrogen fixers, phytohormone producers and biocontrol agents, which are commonly grouped as plant growth promoting bacteria (PGPB). Microbial formulations can be developed using single strain of bacterial isolate, but due to their inconsistency in performance under different field conditions, emphasis has been given towards developing microbial consortium i.e. combination of several PGPB in single carrier. Also, microbial consortium increase the nutrient use efficiency by crops through N2 fixation, solubilization of insoluble phosphate and zinc compounds (Rodriguez and Fraga, 1999; Thakuria et al., 2009), which is crucial tool to overcome phosphorous and zinc fixation in acid soils. So, the objectives of the present studies were (1) Growth curve determination and screening for Plant growth promoting traits of identified bacterial isolates, (2) Determination of shelf-life of biofertilizer formulations in both pure culture and consortium mode, and (3) Comparative performance of pure culture and consortium biofertilizers on growth and yield of apical rooted cutting (ARC) potato crop. Four identified bacterial isolates; (atmospheric N2-fixing isolate Azotobacter chroococcum Um-Azoto II, phosphate complex dissolving isolate Pantoea anthophila Um-PSB1, mineral potash dissolving isolate Arthrobacter nicotianaeUm-KSB1 and zinc complex dissolving isolate Bacillus sp. CJ7-1) were collected from the repository of the Microbial Ecology Lab., CPGS AS, CAU (Imphal), Umiam, Meghalaya. These pure culture isolates were screened for multifaceted PGP traits viz. production of IAA like substances, N2 fixation, dissolution of zinc and potassium complex and dissolution of insoluble phosphate and growth of isolates along pH gradient. All isolates showed IAA produce ability(ranged from 18.95 to 89.26g ml-1 h-1). All four isolates possessed ability to dissolve Ca3(PO4)2 (ranged from 8.91 to 23.19g ml-1 h-1). Only Um-ZnSB1 isolate can dissolve ZnO (SE = 97.48 %) among others. Um-Azoto II isolate was positive for N2 fixation. All isolates could maintain the optimum growth (107 to 109 cfu ml-1) in the acidic pH range of 4.5 to 6.5. Population of viable cells (cfu counts) of each of four PGPB isolates and in consortium recorded at 24 h, 48 h, 72 h, 80 days, 90 days and 120 days after incubation. All four isolates could maintain >108 cfu ml-1 broth till 90 days of storage. For comparative performance of biofertilizers on crop growth and yield, an field experiment was carried out at AICRP, IFS, ICAR-RC NEH region, Meghalaya. ARC potato crop were grown at 39 plots which contain 13 different treatments. The T13treatment which has all four isolates in consortium mode contributed significantly for enhancing plant growth and tuber yield (6.33 tuber nos.plant-1& 10.67 g tuber-1), also has the potential to reduce plant physiological stress compared to rest of the treatments (3.33 to 5.33 tuber nos.plant-1& 5.8 to 9.72 g tuber- 1.). In conclusion, the NPKZn Biofertilizer formulation is recommended for higher productivity of ARC potato through multilocational field trials. | ||
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_aBiofertilizer. _910233 |
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_aAcidic soil. _910234 |
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_aMicrobial NPKZn _910235 |
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_aThakuria, Dwipendra _eMajor Advisor. _99032 |
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_uhttps://krishikosh.egranth.ac.in/handle/1/5810220549 _yOnline |
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_2ddc _cTH |
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_c5685 _d5685 |
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